diff --git a/cibuildwheel/options.py b/cibuildwheel/options.py
index 2d3e32a3..8dd21a1a 100644
--- a/cibuildwheel/options.py
+++ b/cibuildwheel/options.py
@@ -833,9 +833,11 @@ class Options:
                     msg = f"Failed to parse test runtime config. {e}"
                     raise errors.ConfigurationError(msg) from e
 
-            test_requires = self.reader.get(
-                "test-requires", option_format=ListFormat(sep=" ")
-            ).split()
+            test_requires = shlex.split(
+                self.reader.get(
+                    "test-requires", option_format=ListFormat(sep=" ", quote=shlex.quote)
+                )
+            )
             test_extras = self.reader.get("test-extras", option_format=ListFormat(sep=","))
             test_groups_str = self.reader.get("test-groups", option_format=ListFormat(sep=" "))
             test_groups = [x for x in test_groups_str.split() if x]
@@ -932,9 +934,11 @@ class Options:
             )
             audit_command = audit_command_str.split(" && ") if audit_command_str else []
 
-            audit_requires = self.reader.get(
-                "audit-requires", option_format=ListFormat(sep=" ")
-            ).split()
+            audit_requires = shlex.split(
+                self.reader.get(
+                    "audit-requires", option_format=ListFormat(sep=" ", quote=shlex.quote)
+                )
+            )
 
             return BuildOptions(
                 globals=self.globals,
diff --git a/docs/options.md b/docs/options.md
index 2e17043c..542d12ba 100644
--- a/docs/options.md
+++ b/docs/options.md
@@ -1670,6 +1670,10 @@ Platform-specific environment variables are also available:<br/>
     # Install specific versions of test dependencies
     [tool.cibuildwheel]
     test-requires = ["pytest==8.2.2", "packaging==24.1"]
+
+    # Dependency specifiers with environment markers are supported
+    [tool.cibuildwheel]
+    test-requires = ["pytest", "pyzstd; python_version >= '3.14'"]
     ```
 
     In configuration files, you can use an array, and the items will be joined with a space.
@@ -1682,6 +1686,10 @@ Platform-specific environment variables are also available:<br/>
 
     # Install specific versions of test dependencies
     CIBW_TEST_REQUIRES: pytest==8.2.2 packaging==24.1
+
+    # Use shell-style quoting around specifiers containing spaces, such as
+    # those with environment markers
+    CIBW_TEST_REQUIRES: "pytest 'pyzstd; python_version >= \"3.14\"'"
     ```
 
 
diff --git a/test/test_android.py b/test/test_android.py
index 4037b004..59cec2c2 100644
--- a/test/test_android.py
+++ b/test/test_android.py
@@ -405,7 +405,11 @@ def test_environment_markers(tmp_path: Path) -> None:
             **cp313_env,
             "CIBW_TEST_COMMAND": f"python -m pytest {test_filename}",
             "CIBW_TEST_SOURCES": test_filename,
-            "CIBW_TEST_REQUIRES": "pytest certifi;sys_platform=='android' platformdirs;sys_platform!='android'",
+            "CIBW_TEST_REQUIRES": (
+                "pytest"
+                " 'certifi; sys_platform == \"android\"'"
+                " 'platformdirs; sys_platform != \"android\"'"
+            ),
         },
     )
 
diff --git a/unit_test/options_test.py b/unit_test/options_test.py
index 6b586038..aafeb161 100644
--- a/unit_test/options_test.py
+++ b/unit_test/options_test.py
@@ -106,6 +106,35 @@ def test_options_1(tmp_path: Path, monkeypatch: pytest.MonkeyPatch) -> None:
     assert local.pyodide_version == "0.29.4"
 
 
+def test_test_and_audit_requires_with_dependency_specifiers(tmp_path: Path) -> None:
+    """Regression test for https://github.com/pypa/cibuildwheel/issues/2912"""
+    pyproject_toml = tmp_path / "pyproject.toml"
+    pyproject_toml.write_text(
+        """
+[tool.cibuildwheel]
+test-requires = [
+    "pytest",
+    "pyzstd; python_version >= '3.14'",
+    "zarr>=3",
+]
+audit-requires = ["abi3audit; python_version >= '3.9'"]
+"""
+    )
+
+    args = CommandLineArguments.defaults()
+    args.package_dir = tmp_path
+
+    options = Options(platform="linux", command_line_arguments=args, env={})
+    build_options = options.build_options(identifier=None)
+
+    assert build_options.test_requires == [
+        "pytest",
+        "pyzstd; python_version >= '3.14'",
+        "zarr>=3",
+    ]
+    assert build_options.audit_requires == ["abi3audit; python_version >= '3.9'"]
+
+
 def test_passthrough(tmp_path: Path, monkeypatch: pytest.MonkeyPatch) -> None:
     with tmp_path.joinpath("pyproject.toml").open("w") as f:
         f.write(PYPROJECT_1)